Shellab BACTRONEZ Manuale Utente

BACTRON® 110 – 120 Volts Installation and Operation Manual BACTRONEZ

10 | P a g e GRAPHIC SYMBOLS The BACTRONEZ is provided with multiple graphic symbols located on its exterior and interior surfaces. The symbols ide

11 | P a g e GRAPHIC SYMBOLS (CONTINUED) Symbol Definition WEEE Directive compliant logo Indicates the unit should be recycled (Not disposed

12 | P a g e INSTALLATION AMBIENT CONDITIONS This workstation is intended for use indoors, at room temperatures between 15C and 30C (59F and

13 | P a g e INSTALLATION (CONTINUED) HIGH ALTITUDE LOCATIONS BACTRONEZ anaerobic workstations are equipped with two (2) digital vacuum control

14 | P a g e INSTALLATION (CONTINUED) LIFTING AND HANDLING The BACTRONEZ is heavy, and care should be taken to use appropriate lifting devices t

15 | P a g e INSTALLATION (CONTINUED) GAS SOURCE Warning: Never exceed a 5% hydrogen concentration inside the unit’s chamber. Avertissement: La

16 | P a g e INSTALLATION (CONTINUED) WORKSPACE CHAMBER ORIENTATION Figure 1: Workspace Chamber Layout PASS BOX ORIENTATION Figure 2: Pass Box

17 | P a g e INSTALLATION (CONTINUED) CLEANING The BACTRONEZ interior was cleaned and disinfected at the factory, but not sterilized. See the Cl

18 | P a g e CONTROL PANEL OVERVIEW Figure3: Main Control Panel Power Switch The main power switch on the control panel controls all power to th

19 | P a g e CONTROL PANEL OVERVIEW (CONTINUED) Heating Activated Light The clear pilot light located beneath the label HEATING ACTIVATED illum

2 | P a g e BACTRONEZ Anaerobic Workstation 110 - 120 Volts Installation and Operation Manual Part number (Manual): 4861701 Revision: August 27,

20 | P a g e CONTROL PANEL OVERVIEW (CONTINUED) Pass Box Vacuum Controller and Digital Display The main control panel vacuum gauge-controller dis

21 | P a g e CONTROL PANEL OVERVIEW (CONTINUED) VAC Light The clear VAC pilot light indicates that the vacuum pump is in operation when the aut

22 | P a g e OPERATION THEORY OF OPERATION AND MAJOR COMPONENT FUNCTIONS Achieving Anaerobic Conditions No OxygenHydrogenPositive PressureActive

23 | P a g e OPERATION (CONTINUED) Accessing the Workstation Items such as media containers and laboratory equipment can be introduced to or r

24 | P a g e OPERATION (CONTINUED) The Over Temperature Limit System The OTL is a mechanical heating control backup system included with the incuba

25 | P a g e OPERATION (CONTINUED) SETTING UP THE BACTRONEZ Perform the following steps to set up the BACTRONEZ workstation for use: 1. Remove a

26 | P a g e OPERATION (CONTINUED) 7. Perform the following procedures to setup the BACTRONEZ workstation for use. Setup the Gas Supply page 27

27 | P a g e OPERATION (CONTINUED) Figure 12: Gas and Vacuum Ports SET UP THE GAS SUPPLY See the Gas Source entry in the Installation section on

28 | P a g e OPERATION (CONTINUED) SINGLE GAS CONNECTION Follow these steps to connect a single cylinder of anaerobic mixed gas. 1. Procure a T-s

29 | P a g e OPERATION (CONTINUED) CONNECTING TO AN IN-HOUSE VACUUM SYSTEM Carry out this procedure to connect the BACTRONEZ to an in-house vacuu

3 | P a g e TABLE OF CONTENTS INTRODUCTION ...

30 | P a g e OPERATION (CONTINUED) CONNECTING THE FOOT PEDAL ASSEMBLY A foot pedal assembly is required to evacuate and purge the sleeve assemblie

31 | P a g e OPERATION (CONTINUED) SHELVING INSTALLATION Install the three (3) metal bottom shelf spacers included with the BACTRONEZ on the b

32 | P a g e OPERATION (CONTINUED) ESTABLISHING AN ANAEROBIC ENVIRONMENT Carry out the following steps to purge the aerobic atmosphere from the wo

33 | P a g e OPERATION (CONTINUED) Establishing an Anaerobic Environment Continued Note: If you have opened the left armor port door to install

34 | P a g e OPERATION (CONTINUED) INSTALLING THE SLEEVE ASSEMBLIES Prior to verifying the achievement of an anaerobic atmosphere, install the

35 | P a g e OPERATION (CONTINUED) CHAMBER ENTRY Perform these steps to access and work in the workspace chamber without drawing in aerobic at

36 | P a g e OPERATION (CONTINUED) VERIFYING AN ANAEROBIC ATMOSPHERE This procedure verifies that an anaerobic atmosphere has been established pr

37 | P a g e OPERATION (CONTINUED) TROUBLESHOOTING THE PRESENCE OF OXYGEN 1. Verify that proper sleeve donning, entry, and exiting procedure

38 | P a g e OPERATION (CONTINUED) EXITING THE CHAMBER The following steps are used to exit the chamber without pulling in aerobic atmosphere.

39 | P a g e OPERATION (CONTINUED) SETTING THE INCUBATOR TEMPERATURE 1. Using a coin or other flat metal implement, turn the Over Temperature

4 | P a g e Calibrate the Incubator Temperature display ...

40 | P a g e OPERATION (CONTINUED) OPERATING THE PASS BOX The pass box is used to introduce items into or remove items from the workspace chamber

41 | P a g e OPERATION (CONTINUED) Manual Cycle The manual cycle is intended as a backup for use in the event the auto cycle is not functionin

42 | P a g e OPERATION (CONTINUED) LOADING THE WORKSTATION Sheldon Manufacturing recommends waiting 24 hours after establishing an anaerobic atmo

43 | P a g e OPERATION (CONTINUED) CALIBRATE THE INCUBATOR TEMPERATURE DISPLAY Temperature calibrations are performed to ensure that the contro

44 | P a g e OPERATION (CONTINUED) 8. Allow the incubator a minimum of four (4) hour to stabilize after reaching the new temperature. 9. Compa

45 | P a g e OPERATION (CONTINUED) ACTIVATED CHARCOAL SCRUBBER AND REJUVENATION CYCLE An activated charcoal scrubber can be placed in the wo

46 | P a g e OPERATION (CONTINUED) DEIONIZED AND DISTILLED WATER Note: Do not use deionized water for cleaning or humidifying your workstation.

47 | P a g e USER MAINTENANCE Chamber Quality Control Check Sheet Month: Temperature Catalyst Changed Condensate Drained Cylinder Press

48 | P a g e USER MAINTENANCE (CONTINUED) DAILY MAINTENANCE 1. Exchange the catalyst cartridge with a reactivated one. 2. Remove and empty the

49 | P a g e USER MAINTENANCE (CONTINUED) LEAK DIAGNOSIS – UNIT FULL Perform this leak check procedure for a unit that is in use and that cannot

5 | P a g e INTRODUCTION Thank you for purchasing a Shel Lab BACTRONEZ Anaerobic Chamber Workstation. We know that in today’s competitive marketp

50 | P a g e USER MAINTENANCE (CONTINUED) LEAK CHECK – EMPTY UNIT Use this procedure to verify the atmospheric integrity of a BACTRONEZ that is not

51 | P a g e USER MAINTENANCE (CONTINUED) 11. A gas leak detector capable of detecting hydrogen (Part Number 4600501) can be used to find leaks

52 | P a g e USER MAINTENANCE (CONTINUED) REPLACING THE SLEEVE CUFFS A sleeve cuff should be replaced if it shows signs of brittleness or dryness,

53 | P a g e USER MAINTENANCE (CONTINUED) Warning: Disconnect the power cord from the wall power supply prior to cleaning or disinfecting the BAC

54 | P a g e USER MAINTENANCE (CONTINUED) DISINFECTING Disinfect the BACTRONEZ on a regular basis. Perform the following steps to disinfect the w

55 | P a g e USER MAINTENANCE (CONTINUED) MAINTAINING THE ACRYLIC GLASS PANELS Sheldon Manufacturing recommends using Novus brand acrylic glas

56 | P a g e USER MAINTENANCE (CONTINUED) CONDENSATION AND THE DEW POINT Relative humidity inside the BACTRONEZ should never exceed 80% at 25°C.

57 | P a g e UNIT SPECIFICATIONS The BACTRONEZs is a 110 - 120 workstation. Please refer to your workstation’s data plate for individual electri

58 | P a g e UNIT SPECIFICATIONS (CONTINUED) STAND DIMENSIONS Model Inches W × D × H Centimeters W × D × H BACTRONEZ 49 x 30 x 30 inches 124 x 76

59 | P a g e UNIT SPECIFICATIONS (CONTINUED) CHAMBER CAPACITY Workspace Chamber Volume Model Cubic Feet Cubic Liters BACTRONEZ 13.7 388 Workspac

6 | P a g e INTRODUCTION (CONTINUED) ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products. As a result, enginee

60 | P a g e PARTS LIST Description Parts Number Anaerobic Indicator Strips (A box of 100 packets) 9900706 Pass Box Door Gasket 9 x 9, Burgundy (i

61 | P a g e PARTS (CONTINUED) ORDERING PARTS AND CONSUMABLES If you have the Part Number for an item, you may order it directly from Sheldon Ma

62 | P a g e ACCESORIES Shel Lab offers the following BACTRONEZ accessories and kits for sale. Please see our website for prices. The BACTRO

63 | P a g e ACCESSORIES (CONTINUED) Anatox Fan Holds Anatox charcoal filters and significantly speeds the removal of sulfides, fatty acids, and

64 | P a g e ACCESSORIES (CONTINUED) Leak Detector for BACTRONEZ Anaerobic Chambers A handheld gas detector for locating leaks. Recommended for un

7 | P a g e RECEIVING YOUR BACTRONEZ INSPECTING THE SHIPMENT Before leaving our factory, all BACTRONEZs are packaged in high-quality shipping ma

8 | P a g e RECEIVING (CONTINUED) REQUIRED AND USEFUL ACCESSORIES The BACTRONEZ Does not include a vacuum pump, foot pedal assembly, an additiona

9 | P a g e RECEIVING (CONTINUED) RECORDING DATA PLATE INFORMATION Locate the data plate in the workspace chamber above the inner pass box door